What's New in WinLTP 0.96?
Changes to WinLTP (beta
version 0.96) from WinLTP (beta version 0.95b) (September, 2008)
| |
WinLTP096 is
primarily a patch clamp upgrade |
| |
|
Record and stimulate from two patch-clamp electrodes simultaneously
|
| |
|
|
1. |
Add second AnalogOutput channel (IC1) for AnalogInput channel AD1 |
|
|
|
2. |
Enable Rs/Rm analysis on AD1 |
|
|
WinLTP works Windows Vista (with M-Series, but not Digidata
132x boards)
|
|
|
WinLTP Reanalysis works on Macs with Intel processors
|
| |
|
Improved detection
|
| |
|
|
1. |
Implemented Maximum
Slope measurement within a larger BegTime/EndTime range |
|
|
|
2. |
RiseTime and DecayTime Low% to High% of peak fields enabled |
|
|
|
3. |
Latency % of peak field enabled |
|
|
Save your Spreadsheet/ AmpFile data to an Excel XLS file
|
| |
|
Reanalyze straight ASCII files (skip header)
|
|
|
Save relative sizes of panels to type protocol *.pro disk file
|
|
|
Save ADsweep files with/without Time Column
|
|
|
In AboutBox add total physical memory, available physical memory,
percent of memory in use
|
|
|
When running M-Sseries board, check that processor is HyperThreading or
multi-Core
|
|
|
When running Digidata board, warn if processor is < 2.8 GHz
|
|
|
Start in Protocol Scripting mode instead of LTP Protocol mode
|
| |
|
Bug fixed in Rs/Rm Measurement due to faulty use of RsRm pulse
amplitude
|
| |
|
|
1. |
When a protocol file is loaded, the RsRm pulse amplitude is the one
used CORRECTLY for Rs and Rm calculations. |
|
|
|
2. |
Therefore, if you don’t change your RsRm pulse amplitude during
experiments, only the first experiment after a new protocol file has been written may have incorrect Rs and Rm values. The rest of the experiments will be
fine. |
|
|
|
3. |
However, if this RsRm pulse amplitude is changed during the experiment, the
original RsRm amplitude is INCORRECTLY still used for all Rs and Rm
calculations in this experiment. This will result in Rs and Rm being both incorrect and even
negative for this one experiment. |
|
|
Zooming in ADsweep and Analysis graphs now works well
|
|
|
Y CalibrationBar in EnhancedMetafile is now of reasonable length (one NumberLabeled GridLine).
|
| |
|
FilesToReanalyze - Filename template now sticks from one analysis to another
|
|
|
DecayTimes were in minus msecs, now they are in plus msecs
|
|
|
Rapidly resizing the Protocol/Detection panel and the Mainpg panel no
longer causes the program to hangup
|
|
|
The video related bug
with Windows XP no longer occurs (before WinLTP could hang up during
start-up if the XP font size was Large or Extra Large rather than
Normal).
|
Changes to WinLTP (beta version 0.95b) from WinLTP (beta
version 0.95) (September, 2007)
| |
This is basically a time
limit extension version. |
| |
|
WinLTP095b will now work
with National Instruments M-Series boards using the PCIexpress bus.
|
Changes to WinLTP (beta version 0.95) from WinLTP (beta
version 0.94) (February, 2007)
| |
LTP Protocol
enhancements (no scripting is involved)
|
| |
|
Bug Fixed: When
Single and Repeat PulseSweeps are evoked during an AvgLoop, they will
not be run during the AvgLoop, but only after it is finished.
|
|
|
For LTD stimulation with averaging (or any AvgLoop within a Loop), now
print the current number of sweeps evoked and total number of sweeps to
be evoked in the RunLine.
|
|
|
After an evoked
FastRepeat LTD or ThetaBurst stimulation, an extra Delay period can be
added to separate this stimulation from the subsequent normal repeating
sweep stimulation.
|
| |
Protocol
Scripting enhancements
|
| |
|
An evoked single or
repeat sweep stimulation can occur at beginning of a Delay
statement. This allows one to precisely place when an evoked sweep
will occur during a delay period.
|
|
|
Run Once[x] checkbox so that you do not need to uncheck a [ ]Run
statement when you want to run the the code within the Run construct
only once
|
|
|
Run / ElseRun
construct to quickly change between one or the other of two stimulations
in Run statements
|
| |
|
Implement Enable Single & FastRepeat CheckBoxes so that only
the buttons for the evoked Single or Repeat stimulations you want will
be enabled
|
| |
The WinLTP Manual
has been essentially completed |
| |
|
The Reanalysis chapter has been completed
|
| |
|
Evoking Single or Repeat
Pulse Sweeps (P0sweep or P1sweep) can no longer occur in an AvgLoop
|
| |
|
|
1. |
In the previous versions of WinLTP from WinLTP090 to WinLTP094, this
was because in an Averaging Loop the P0sweeps and the P1sweeps are being
averaged by adding to an array in
each pulse sweep that sums all the sweeps and then is divided by the
number of loops. If Single
or Repeat P0 or P1 sweeps are evoked, this adds to the sum array but
does not change the number of loops – hence the average will be wrong! |
|
|
|
2. |
However, evoking Single or Repeat T0 or T1 sweeps in an Averaging Loop
is fine because these do not have a sum array and averaging is never
done on them. And if you are
doing scripting, only one P0sweep and only one P1sweep is allowed in an
Averaging Loop – e.g. this possible bug has been prohibited in
scripting.. |
|
|
|
3. |
This bug was fixed by
changing WinLTP so that when Single and Repeat PulseSweeps are evoked
during an AvgLoop, they will not be run during the AvgLoop, but only
after it is finished |
| |
|
Blocked inserting an AvgLoop within an AvgLoop (since it doesn't make
sense to do so)
|
|
|
Fixed an 'Access
Violation in module Tee6c6.bpl’ error in the Reanalysis program that
occurred when averaging trains with analyzing all pulses in the train
|
Changes to WinLTP (beta version 0.94) from WinLTP (beta
version 0.93) (Primarily a debug version) (October, 2006)
| |
This version is
primarily a bug fix version due to the change in calculating Rm. |
| |
|
Corrected Rm cell
input resistance measurement bug
|
| |
|
|
In the previous versions
of WinLTP (WinLTP090 to WinLTP093), the cell input resistance Rm was incorrectly
calculated as
|
|
|
|
Rm = VPulse / ISteadyState
|
|
|
|
and is now correctly calculated in WinLTP094 and later as
|
|
|
|
Rm = VPulse
/ ISteadyState - Rs
|
|
|
|
Because Rs is usually
much less than Rm, Rm ~
Vpulse / Isteadstate still
is roughly true, but since Rs values are typically 5% to 10% of Rm
values, previous Rm values (from WinLTP090 to WinLTP093) are roughly 5%
to 10% too high. Since Rm = Vpulse / Isteadystate - Rs
is the theoretically correct function, it is now used in the Rm
calculation when Rs is being measured.
|
| |
Fixed M-Seres board
bug where input signals "wrap around" when <-10V or
>+10V |
| |
|
Input signals are now correctly pinned at -10V or +10V.
|
| |
Changed the Rm
measurement from 50%->90% to 70%->90% of the
RsRm test pulse duration |
| |
|
I found that sometimes
the voltage had not settled to steady state by 50% of the pulse duration
and therefore increased it to 70% in hopes of obtaining more accurate Rm
measurements.
|
| |
Added an 's' after
the Sweep and Delay Period fields for clarity, to denote their
measurements in seconds |
| |
|
For example, changed
[ ]P0sweep 20 and
[ ]Delay 10 to
[ ]P0sweep 20s and
[ ]Delay 10s.
|
Changes to WinLTP (beta version 0.93) from WinLTP (beta
version 0.92) (August, 2006)
| |
WinLTP now supports the National Instruments M-Series boards for
the PCI bus
|
| |
|
Supported M-Series boards include the PCI-6221, PCI-6229, PCI-6251 and
PCI-6259
|
|
|
M-Series boards for the USB bus are currently not yet supported
|
| |
Decrease in response time delay to keyboard input for M-Series PCI
boards
|
| |
|
The response time delay to keyboard input has decreased from 5.0 sec
for Axon Digidata 132x boards to 0.5 sec for M-Series PCI boards
|
Changes to WinLTP (beta version 0.92) from WinLTP (beta
version 0.91) (a debug version) (March, 2006)
This is a last minute debug version before I begin
installing the National Instruments M-Series boards.
| |
Fixed PowerOn code bug - there is no longer any 5v digital output
|
| |
|
Now when the Digidata 1320A/1322A is tuned on, all digital and and analog
outputs go to 0 volts
|
|
|
If somehow the Power-On data changes, WinLTP will recognize this and
require recalibration of the Digidata 1320A/1322A
|
| |
CalcGraph MinTime values now snap to 1 min increments
|
| |
|
Previously, CalcGraph MinTime values snapped to values 1/10the the
plot duration. If the MinTime was say set to 5 min, and the plot
duration was 300 minutes, then clicking on the CalcGraph with the mouse
could cause the MinTime to snap back to 0 min, which was usually not
desired. This no longer happens.
|
| |
When the Reanalysis dialog box is reopened, the previous last
FilenameTemplate used is now shown
|
| |
|
This has been reworked and now hopefully works correctly
|
| |
We have apparently solved the
problems with the Digidata 1320A
|
| |
|
The problem with getting certain (probably older)
Digidata 1320As to install, calibrate and work properly is at
least partly due to using old AxoScope dll files. If you
are having problems, upgrade your AxoScope to the lastest version (and
copy over the dll files to the \ WinLTP folder) by freely downloading
AxoScope from Molecular Devices at:
|
Changes to WinLTP (beta version 0.91) from WinLTP (beta
version 0.90) (Primarily a debug version) (February, 2006)
| |
Improved Reanalysis program
|
| |
|
Now ADsweep and Calc graphs properly clear
when reanalyzing at high speed with no delay between files loaded (e.g.
if the Reanalysis Period = 0 sec)
|
| |
When the Reanalysis dialog box is reopened, the previous last
FilenameTemplate used is now shown
|
|
|
When the Reanalysis dialog box is reopened, the previous last
FilenameTemplate (e.g. *.P1) and FilesOfType (Pulse Files (*.P?)) are
now shown.
|
| |
|
In other words, you
don’t have to retype! the FilenameTemplate or FilesOfType each
time you reanalyze!
|
| |
The improved ability to run continuous sweeps (with no delay between
them)
|
| |
|
In WinLTP090 this was a Limitation as described below as:
|
| |
|
|
“Probably the most computer intensive task that WinLTP has to face
is repeat sweep stimulation with no delay in-between.
For now, a 3.2GHz Pentium 4 processor and a fast video graphics
card has difficulty saving contiguous sweeps longer than 2 seconds. (This is
because, for now, sweeps are saved after, rather than during,
acquisition.) Contiguous
sweeps of down to 0.1 sec duration, however, can be saved." |
| |
|
This Limitation has now been largely removed
|
| |
|
|
Now a 3.2GHz Pentium 4 computer with a fast video graphics card can
run and save contiguous sweeps of 100
seconds. |
| |
|
To remove this Limitation these changes were made:
|
|
|
|
1. |
ContAcq graphs now always correctly clear |
|
|
|
2. |
ADsweeps and Analysis Graphs now always correctly plot |
|
|
|
3. |
The time to next sweep is now correctly printed on the RunLine |
| |
|
|
4. |
Tabs, Dialogs, Fields and StimGraphs are now quickly updated
|
| |
Save ADsweep Graph as a Windows Enhanced Metafile
|
| |
|
This allows ADsweep graphs to be directly imported into plotting
programs. Just double click
(using the left mouse button) on the dark gray area containing the axis
values surrounding the ADsweep data window to bring up the metafile
dialog box (see Section 9.6 of the WinLTP Getting Started manual).
|
| |
CalcLines, Brackets, Crosses and Arrows are now plotted on ADsweep
graphs
|
| |
|
These now more clearly show where
|
|
|
|
1. |
Duration is marked by Vertical Lines and Left/Right Arrows |
|
|
|
2. |
PSamp is marked by Up/Down Arrows and Left/Right Brackets |
|
|
|
3. |
PkAmp is marked by Up/Down Arrows |
|
|
|
4. |
Coastline is marked by Left/Right Brackets |
| |
|
|
5. |
RiseTime & DecayTime are marked by Crosses
|
| |
Added ElectrodeConnections dialog box
|
| |
|
This is opened from the Help menu, and shows where the stimulus
isolation units and amplifiers are connected to the data acquisition
board.
|
Changes to the
beta version of WinLTP (version 0.90) from the DOS LTP Program (version 2.4)
(August, 2005)
| |
|
LTP Program capability (repeat sweep stimulation, acquisition and
analysis) maintained and advanced
|
| |
|
Tape recorder (Continuous Acquisition of 2 AD channels down to 25 usec
sample intervals, saved to a gap-free Axon Binary File) |
| |
|
Capture Spontaneous Events and save to disk (Online analysis not implemented yet) |
| |
|
| |
Dynamic Script Programming for writing complex protocols.
No programming required for LTP protocols.
|
|
|
|
Repetitive sweeps sampling interval now down to 25 usec
|
|
|
| |
Fast Repeat (LTD) Sweep Stimulation with no time between sweeps
|
| |
|
| |
Patch clamp stimulation now in mV |
| |
|
| |
Patch Electrode Series Resistance (Rs) and Cell Resistance (Rm) now
measured in Mohms |
|
More accurate Patch Electrode Series Resistance (Rs) (using lower 50
or 25 usec sample intervals) |
| |
|
| |
Runs in Windows 2000 and XP
|
Return to the WinLTP Home Page