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| Note: |
If you are using
WinLTP 1.00 you will have to upgrade to this version of WinLTP,
1.01. |
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However, you
will NOT have to rewrite protocol files. |
| We are announcing that
running WinLTP Acquisition in the Advanced Mode (to use Protocol
Scripting) now requires the purchase
of a License Key.
However, the running WinLTP Acquisition
in the Basic Mode (to use the simpler LTP Protocol) continues to
be free and no longer has any time out code. (Currently,
there are no plans for to charge in future versions of WinLTP for running in the
Basic Mode. However future changes
to this policy cannot be eliminated.)
Running WinLTP Reanalysis will
continue to be free.
You will now never have to write another
protocol file unless you decide to upgrade to a newer version of WinLTP.
In order to sell the permanent, Advanced
Mode License Key, we
have started a company, WinLTP Ltd., in agreement with The University
of Bristol. The introductory cost for the License Key
is $1000 for 5 copies.
We hope that you will support WinLTP by
purchasing an Advanced Mode License. This helps us to provide
technical support of Basic Mode users throughout the world, and to
continue further WinLTP development.
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WinLTP is a stimulation, data acquisition and on-line
analysis electrophysiological software for studying Long-Term Potentiation (LTP), Long-term
Depression (LTD), and related phenomena. WinLTP is multitasking and
simultaneously runs
1) LTP stimulus/acquisition/analyzing sweeps with protocol scripting,
and 2) continuous acquisition saving Axon Binary Files (abf).
WinLTP runs
on Windows PCI bus computers
and uses National Instruments PCI M-Series boards and Axon Instruments' Digidata
1320A and 1322A data acquisition boards.
Other software that can use the M-Series boards includes Axograph
Scientific's AxoGraph X, WaveMetrics' IGOR,
National Instruments' LabView, John Dempster's Strathclyde Electrophysiology
Suite (WinWCP
and WinEDR), Silver lab's Nclamp,
and QUB data acquisition.
The basic design philosophy behind WinLTP is to provide
simple stimulation protocols using the LTP Protocols in the Basic Mode, or more complex
stimulation protocols using Protocol Scripting in the Advanced mode, do sufficient online analysis to
let you modify the experiment as it runs (such as changing baselines or protocol
flow), do simultaneous continuous acquisition, and use inexpensive but excellent
data acquisition boards (National Instruments M-Series boards).
WinLTP
on/off-line analyses include basic analyses of synaptic potentials (Peak Amplitude, Latency, Slope, Area, Duration, Rise/Decay Time,
Coastline, PopSpike
Amplitude and Latency, Average Amplitude), and also Cell Resistance (Rm), and
Patch Electrode Series Resistance (Rs).
However, WinLTP is not designed to
do every possible synaptic analysis, and instead is designed to work with other
very good, and often inexpensive analysis programs. For example,
additional synaptic event analyses such as synaptic exponential decay time can
be done by separate analysis programs including Synaptosoft's MiniAnalysis
and Christoph Schmidt-Hieber's StimFit32,
and spontaneous synaptic events in continuous acquisition gap-free abf
files can be analyzed by William Heitler's DataView,
Synaptosoft's MiniAnalysis, Axograph
Scientific's AxoGraph X and Molecular
Device's ClampFit.
If you have any questions please contact WinLTP
support at: support@winltp.com.
| More Complex WinLTP functionality |
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1. |
Multitasking |
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a. Repeat sweep stimulation, acquisition and
analysis
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b. Tape recorder (Continuous Acquisition of 2 AD channels down
to 25 usec sample intervals, saved to a gap-free Axon Binary File)
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2. |
Dynamic Protocol Scripting for writing complex protocols.
No programming required for basic LTP Protocols.
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3. |
Fast Repeat (LTD) Sweep Stimulation with no time between sweeps
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4. |
On and off-line calculation and plotting of several
waveform parameters: DC baseline, Peak Amplitude, Latency, Slope, Maximum
Slope, Area, Duration, Rise Time, Decay Time, Coastline, PopSpike
Amplitude, PopSpike Latency, Average Amplitude, Cell
resistance (Rm), and Patch electrode series resistance (Rs) |
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5. |
Analyze all S0- and S1-evoked postsynaptic responses in
a
sweep
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6. |
Special analyses of trains including: Analyze all peaks in a train
relative to the baseline of the first pulse
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7. |
Automatic blanking of stimulus artifacts
to allow accurate determination of peaks and areas in a train
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8. |
Measurement of Patch Electrode Series Resistance (Rs)
using Rs peak, or Rs
single or double exponential curve fitting |
| Simple WinLTP functionality |
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1. |
The WinLTP records synaptic activity in
extracellular, intracellular or patch clamp modes |
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2. |
2 AD channel acquisition (down to 25 usec sample interval)
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3. |
Two extracellular stimulation outputs (S0 and S1) |
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4. |
Two simultaneous patch-clamp recordings using two analog
outputs |
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5. |
Analog
stimulation including analog
trains and ramps (loop within loop stimulation) |
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6. |
Repetitive sweeps with simultaneous data acquisition (up
to 1,000,000 samples and 100 sec duration) and stimulation (using two
extracellular pathway stimulation, S0 and/or S1, and epoch-like digital
and intracellular analog stimulation) |
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7. |
Basic LTP Protocols are either slow single pathway S0
stimulation, or slow alternating dual pathway (S0 then S1) stimulation |
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8. |
The sweep data can be signal averaged and digitally
filtered on-line and off-line |
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9. |
LTP induction can be produced by: Single train, repetitive train (theta burst stimulation), and
primed
burst stimulation |
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LTD stimulation and analysis can be performed using fast
repetitive single pulse sweeps (at up to 10 Hz), or several pulses in a
sweep for faster repetitive stimulation |
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11. |
Patch
sealtest protocol implemented
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12. |
Save ADsweep Graph as a Windows Enhanced Metafile
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13. |
Save your Spreadsheet/ AmpFile data to an Excel XLS file
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14. |
Reanalyze straight ASCII files (skip header)
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15. |
Automatic data folder creation at startup
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16. |
WinLTP Reanalysis works on Macs with Intel
processors
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17. |
On-line acquisition runs in Windows XP and Vista
(M-Series boards) and Windows 2000 and XP (Digidata 132x boards)
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1. |
For Digidata 132x boards, a PCI bus computer or laptop computer (3 GHz or
higher processor
recommended) |
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For M-Series boards, PCI bus computer with at least a
2.8 GHz Pentium 4 processor (e.g. with hyperthreading, the faster the
better, multi-core processors supported) (M-Series USB boards also
supported) |
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2. |
For M-Series boards, Windows XP or Vista; for Digidata 132x boards, Windows 2000 or XP |
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3. |
2048 MB of memory recomended (512 MB minimum) |
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4. |
1280x1024 pixel monitor recommended (1024x768 minimum) |
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5. |
Data acquisition boards |
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a. |
Molecular Devices' Digidata 1320A or 1322A (Note: NI
PCI boards work much better than Digidata boards) |
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b. |
National Instruments M-Series
PCI boards (PCI-6221, PCI-6229, PCI-6251 or PCI-6259) |
The free DOS LTP Program
is still available (although the free Basic Mode of WinLTP does everything that
the LTP Program does, and more!).
Copyright © WinLTP Ltd. and The University of
Bristol, 1991-2009. All Rights Reserved.
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